Nitrocellulose membrane which side

The 0. See the Blotting Membrane and Filter Paper Selection Guide for more information on the different membrane types and size selections by gel type. This product contains electrical or electronic material.

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However, please note that nitrocellulose requires the use of methanol in the transfer buffer which may reduce the pore size of the gel and cause high molecular weight proteins to precipitate. Physical characteristics. While nitrocellulose is brittle and fragile, PVDF is more durable and has higher chemical resistance making it ideal for reprobing and sequencing applications. Nitrocellulose can prove to be difficult to strip and reprobe without losing signal.

However, supported versions of nitrocellulose membranes are considerably more durable and resilient than standard nitrocellulose membranes so they can be stripped, reprobed, and subjected to harsh chemical treatments. Pore size. Both membranes come in typical pore sizes of 0. The 0. Note: When you are detecting a protein loaded at low levels or when quantification is considered critical, you should always choose the smaller size membrane.

Membrane format. There are several factors that you need to take into account when choosing the most suitable membrane format, including transfer system semi-dry, wet or fast , convenience, price and flexibility.

Pre-cut and pre-wetted membranes are the ideal choice when convenience, reproducibility and high throughput are of highest importance while rolls offers more flexibility since you can cut the membrane to the specific size of your gel. The only problem is that this can add extra time to your workflow and introduce variability in the membrane size.

Pre-cut membranes, on the other hand, are available in a range of sizes suitable for all gel types. Using a precut membrane may result in better transfer reproducibility.

While both nitrocellulose and PVDF membranes are used for Western blotting and amino acid analysis, nitrocellulose is ideal in detecting low molecular weight proteins while PVDF is more suitable for detecting higher molecular weight proteins.

Keep in mind that you can get more reliable results from your Western blot experiment by choosing the correct membrane type. So, choose carefully and you will surely get the results that you need. Image Source : Robert Couse-Baker. Topics: Western Blotting. This assay is suitable for the simple and rapid estimation of protein concentration.